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Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene

热3 cflikunlun 添加于 2010-4-6 20:21 | 2592 次阅读 | 2 个评论

作者
KO C, WOO Y, LEE D, LEE M, KIM C
摘要
We conducted a genetic yeast screen to identify Thermo-tolerance genes (TTOs) in maize kernel cDNA library. During the screening, we identified a maize clone (TTO6) that seemed to confer elevated heat tolerance in comparison to control cells. TTO6 cDNA (GenBank accession no. AY103785) encodes an 11-kDa protein which is 69% similarity to the Arabidopsis GASA4 gene. To further examine heat tolerance in Arabidopsis, we functionally characterized the GASA4 gene and found that heat induced GASA4 expression. Constitutive expression of GASA4 in Arabidopsis led to elevated heat tolerance in transgenic lines. Interestingly, endoplasmic reticulum chaperone expression analysis suggests that GASA4 influences BiP gene expression during heat stress.
详细资料
- 文献种类: Journal Article
- 期刊名称: Plant Physiology and Biochemistry
- 期刊缩写: Plant Physiology and Biochemistry
- 期卷页: 2007年第45卷第9期 722-728页
- ISBN: 0981-9428
学科领域生物医药 » 生物学
标签

GASA4
相关链接 DOI URL
附件
Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene

cflikunlun 的文献笔记订阅

【原创】本文是第一篇发现GASA基因家族的成员-GASA4 基因与非生物胁迫（热）有关的论文，通过对拟南芥超表达进行的功能验证，将提高了超表达拟南芥的耐热性的原因推测为超表达后，使与耐热相关的位于ER的伴侣蛋白BIP的积累，这个推测我觉得并不严格，首先这个来自玉米的GASA4 基因并未经实验验证是定位于ER的，作者将该基因与ER联系在一起的原因只是因为有文献报道来自非洲菊的GASA基因位于ER或分泌到细胞外，另外，在BIP和GASA4 提高抗热性之间肯定不是简单的直接调控的关系，二者之间还有哪些成员以及具体的作用机制还需要进一步设计实验证明

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