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Genome-Wide Kinetics of Nucleosome Turnover Determined by Metabolic Labeling of Histones

xianglanxue 添加于 2010-8-26 14:29 | 1457 次阅读 | 0 个评论

作者
Deal RB, Henikoff JG, Henikoff S
摘要
Nucleosome disruption and replacement are crucial activities that maintain epigenomes, but these highly dynamic processes have been difficult to study. Here, we describe a direct method for measuring nucleosome turnover dynamics genome-wide. We found that nucleosome turnover is most rapid over active gene bodies, epigenetic regulatory elements, and replication origins in Drosophila cells. Nucleosomes turn over faster at sites for trithorax-group than polycomb-group protein binding, suggesting that nucleosome turnover differences underlie their opposing activities and challenging models for epigenetic inheritance that rely on stability of histone marks. Our results establish a general strategy for studying nucleosome dynamics and uncover nucleosome turnover differences across the genome that are likely to have functional importance for epigenome maintenance, gene regulation, and control of DNA replication.
详细资料
- 文献种类:期刊
- 期刊名称: Science
- 期刊缩写: Science
- 期卷页: 2010年第328卷第5982期 1161-1164页
- ISBN: 0036-8075
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