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Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit

jsllgs 添加于 2010-9-26 16:22 | 2175 次阅读 | 0 个评论

作者
Akiyoshi B, Nelson CR, Ranish JA, Biggins S
摘要
The kinetochore is a macromolecular complex that controls chromosome segregation and cell cycle progression. When sister kinetochores make bioriented attachments to microtubules from opposite poles, the spindle checkpoint is silenced. Biorientation and the spindle checkpoint are regulated by a balance between the Ipl1/Aurora B protein kinase and the opposing activity of protein phosphatase I (PP1). However, little is known about the regulation of PP1 localization and activity at the kinetochore. Here, we developed a method to purify centromere-bound kinetochores and used quantitative proteomics to identify the Fin1 protein as a PP1 regulatory subunit. The Fin1/PP1 complex is regulated by phosphorylation and 14-3-3 protein binding. When Fin1 is mislocalized, bipolar spindles fail to assemble but the spindle checkpoint is inappropriately silenced due to PP1 activity. These data suggest that Fin1 is a PP1 regulatory subunit whose spatial and temporal activity must be precisely controlled to ensure genomic stability.
详细资料
- 文献种类:期刊
- 期刊名称: Genes & Development
- 期刊缩写: Genes Dev
- 期卷页: 2009年
- 地址: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
- ISBN: 1549-5477
标签

PP1 有丝分裂
相关链接 DOI URL

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