新科学想法 文献管理 浏览文献

有读书笔记Adult lung side population cells have mesenchymal stem cell potential

Seedson 添加于 2011-12-18 21:34 | 1725 次阅读 | 0 个评论
  •  作 者

    Martin J, Helm K, Ruegg P, Varella-Garcia M, Burnham E, Majka S
  •  摘 要

    BACKGROUND: The development of stem cell therapy for pulmonary diseases remains a challenge. Many diverse cell types reside within the lung and a common stem cell has not yet been identified. A basic understanding of lung stem cell fate during disease may prove important for drug intervention as well as autologous therapies. Niches for resident mesenchymal stem cells (MSC) have been identified in many adult tissues and more recently in the lung. We present data to confirm the observation that non-hematopoietic CD45(neg) lung side population (SP) cells contain MSC, single cells capable of multilineage differentiation. METHODS We carried these observations forward by analyzing the MSC potential of single-cell clones, as well as their chromosomal stability and telomerase activity. RESULTS: The expression of MSC markers was characterized in mouse CD45(neg) lung SP by flow cytometry on freshly isolated or cultured clonal populations. The karyotype of these cells was subsequently assayed by banding analysis, and telomerase activity was assessed using quantitative polymerase chain reaction. MSC differentiation potential was confirmed by the characteristic ability of single-cell clones to differentiate into cells of three mesenchymal lineages, chondrocytes, adipocytes and osteocytes. Differentiation was confirmed by histochemical analysis. All analyzed populations of CD45(neg) lung SP expressed mesenchymal markers (CD44, CD90, CD105, CD106, CD73 and Sca-I) and lacked hematopoietic markers (CD45, c-kit, CD11b, CD34 and CD14). The cultured and clonal CD45(neg) lung SP had normal chromosomal structures and expressed high levels of telomerase. After being expanded and cultured in differentiation medium, all populations of CD45(neg) lung SP demonstrated adipogenic, osteogenic and chrondrogenic potential. Adult CD45(neg) lung SP cells are a source of MSC. DISCUSSION: In defining this tissue-specific stem cell population in the lung, we are now better able to clarify a potential role for them in lung diseases.
  •  详细资料

    • 文献种类:期刊
    • 期刊名称: Cytotherapy
    • 期刊缩写: Cytotherapy
    • 期卷页: 2008  10 2 140-151
    • 地址: Division of Cardiology, Cardiovascular Pulmonary Laboratory, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    • ISBN: 1465-3249
    • 备注:PMID:18368593
  •  标 签

  • 相关链接 DOI URL 

  •  Seedson 的文献笔记  订阅

    成人肺旁侧细胞群具有间质干细胞特性

    BACKGROUND:

    The development of stem cell therapy for pulmonary diseases remains a challenge. Many diverse cell types reside within the lung and a common stem cell has not yet been identified. A basic understanding of lung stem cell fate during disease may prove important for drug intervention as well as autologous therapies. Niches for resident mesenchymal stem cells (MSC) have been identified in many adult tissues and more recently in the lung. We present data to confirm the observation that non-hematopoietic CD45(neg) lung side population (SP) cells contain MSC, single cells capable of multilineage differentiation. METHODS We carried these observations forward by analyzing the MSC potential of single-cell clones, as well as their chromosomal stability and telomerase activity.

    成人肺组织间质干细胞niches被发现。

    RESULTS:

    The expression of MSC markers was characterized in mouse CD45(neg) lung SP by flow cytometry on freshly isolated or cultured clonal populations. The karyotype of these cells was subsequently assayed by banding analysis, and telomerase activity was assessed using quantitative polymerase chain reaction. MSC differentiation potential was confirmed by the characteristic ability of single-cell clones to differentiate into cells of three mesenchymal lineages, chondrocytes, adipocytes and osteocytes. Differentiation was confirmed by histochemical analysis. All analyzed populations of CD45(neg) lung SP expressed mesenchymal markers (CD44, CD90, CD105, CD106, CD73 and Sca-I) and lacked hematopoietic markers (CD45, c-kit, CD11b, CD34 and CD14). The cultured and clonal CD45(neg) lung SP had normal chromosomal structures and expressed high levels of telomerase. After being expanded and cultured in differentiation medium, all populations of CD45(neg) lung SP demonstrated adipogenic, osteogenic and chrondrogenic potential. Adult CD45(neg) lung SP cells are a source of MSC.

    方法:

    1.端粒酶长度测定(即时PCR);

    2.流式细胞测定细胞标记物(有间叶细胞标记物而无造血细胞标记物);

    3.原细胞分化3种细胞系(免疫组化分析)

    DISCUSSION:

    In defining this tissue-specific stem cell population in the lung, we are now better able to clarify a potential role for them in lung diseases.

管理选项: 导出文献

评论(0 人)

facelist doodle 涂鸦板

Copyright;  © 新科学想法 2016-2017   浙公网安备 33010202000686号   ( 浙ICP备09035230号-1 )