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Aurora A is involved in central spindle assembly through phosphorylation of Ser 19 in P150Glued

热1 阿平添加于 2013-4-5 23:01 | 8773 次阅读 | 1 个评论

作者
Reboutier D, Troadec M-B, Cremet J-Y, Chauvin L, Guen V, Salaun P, Prigent C
摘要
Knowledge of Aurora A kinase functions is limited to premetaphase events, particularly centrosome maturation, G2/M transition, and mitotic spindle assembly. The involvement of Aurora A in events after metaphase has only been suggested because appropriate experiments are technically difficult. We report here the design of the first human Aurora A kinase (as-AurA) engineered by chemical genetics techniques. This kinase is fully functional biochemically and in cells, and is rapidly and specifically inhibited by the ATP analogue 1-Naphthyl-PP1 (1-Na-PP1). By treating cells exclusively expressing the as-AurA with 1-Na-PP1, we discovered that Aurora A is required for central spindle assembly in anaphase through phosphorylation of Ser 19 of P150Glued. This paper thus describes a new Aurora A function that takes place after the metaphase-to-anaphase transition and a new powerful tool to search for and study new Aurora A functions.
详细资料
- 文献种类:期刊
- 期刊名称: The Journal of Cell Biology
- 期刊缩写: J Cell Biol
- 期卷页: 2013年第201卷第1期 65-79页
- 地址: Unite Mixte de Recherche 6290, Centre National de la Recherche Scientifique, F-35043 Rennes, France
- ISBN: 0021-9525
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Aurora A 居然还有这个功能，可以通过磷酸化P150Glued将其释放到central spindle上，并且促进central spindle形成。但是为什么在metaphase的时候它就不释放而将大量P150Glued留在centrosome上面呢？难道这时候有个phosphatase有那么活跃？