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miR-301a is a candidate oncogene that targets the homeobox gene Gax in human hepatocellular carcinoma

wanyair 添加于 2013-6-7 17:30 | 1582 次阅读 | 0 个评论

作者
Zhou P, Jiang W, Wu L, Chang R, Wu K, Wang Z
摘要
BACKGROUND: MicroRNAs (miRNA) are a group of noncoding small RNAs that repress mRNA expression or induce mRNA degradation by binding to the 3\'-untranslated regions of mRNAs. MiRNAs have been connected closely with the development of cancers such as hepatocellular carcinoma (HCC). However, the overexpression of microRNA-301a (miR-301a) has seldom been connected with tumorigenesis in HCC. AIMS: This study aims to characterize the function of upregulated miR-301a in HCC and show how the downstream growth arrest-specific homeobox (Gax) is negatively regulated by miR-301a. METHODS: The expression of miR-301a and Gax was detected using real-time PCR on HCC tissues and adjacent non-tumorous tissues. The luciferase reporter assay was used to assess Gax as a target of miR-301a. The nuclear factor kappaB (NF-kappaB) was measured by western blot after inhibiting miR-301a and enhancing Gax. The functions of miR-301a in vivo in HCC cells were measured by migration and invasion assays and flow cytometry. RESULTS: MiR-301a was significantly upregulated and Gax was downregulated in HCC samples compared with in the matching nontumoral tissues. Inhibiting miR-301a expression caused the upregulation of Gax and repressed NF-kappaB expression. We have shown that miR-301a plays an important role in increasing proliferation, migration and invasion and in inhibiting apoptosis of HCC cells. CONCLUSIONS: miR-301a is frequently upregulated in HCC and modulates NF-kappaB expression by negatively regulating Gax.
详细资料
- 关键词: 3\' Untranslated Regions/genetics; Adult; Aged; Apoptosis/*genetics; Carcinoma, Hepatocellular/*genetics; Cell Line, Tumor; Cell Proliferation; Cell Transformation, Neoplastic/genetics; Female; Gene Expression Regulation, Neoplastic; Genes, Homeobox; Homeodomain Proteins/*genetics; Humans; Liver Neoplasms/*genetics; Male; MicroRNAs/*genetics; Middle Aged; NF-kappa B/genetics; *Oncogenes; RNA, Messenger/genetics; Real-Time Polymerase Chain Reaction; Up-Regulation/genetics
- 文献种类:期刊
- 期刊名称: Digestive Diseases and Sciences
- 期刊缩写: Dig Dis Sci
- 期卷页: 2012年第57卷第5期 1171-1180页
- 地址: Department of General Surgery, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, China. zpspp@yahoo.com.cn
- ISBN: 0163-2116
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