新科学想法 文献管理 浏览文献

Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E

聚焦生物 添加于 2015-8-2 22:22 | 1428 次阅读 | 0 个评论
  •  作 者

    Kim Y, Holland AJ, Lan W, Cleveland DW
  •  摘 要

    Opposing roles of Aurora kinases and protein phosphatase 1 (PP1) during mitosis have long been suggested. Here, we demonstrate that Aurora kinases A and B phosphorylate a conserved residue on the kinetochore motor CENP-E. PP1 binds CENP-E via a motif overlapping this phosphorylation site and binding is disrupted by Aurora phosphorylation. Phosphorylation of CENP-E by the Auroras is enriched at spindle poles, disrupting binding of PP1 and reducing CENP-E\'s affinity for individual microtubules. This phosphorylation is required for CENP-E-mediated towing of initially polar chromosomes toward the cell center. Kinetochores on such chromosomes cannot make subsequent stable attachment to spindle microtubules when dephosphorylation of CENP-E or rebinding of PP1 to CENP-E is blocked. Thus, an Aurora/PP1 phosphorylation switch modulates CENP-E motor activity as an essential feature of chromosome congression from poles and localized PP1 delivery by CENP-E to the outer kinetochore is necessary for stable microtubule capture by those chromosomes.
  •  详细资料

    • 关键词: Amino Acid Sequence; Animals; Aurora Kinases; Chromosomal Proteins, Non-Histone/*metabolism; Chromosomes/*metabolism; HeLa Cells; Humans; Kinetochores/metabolism; Microtubules/metabolism; Molecular Sequence Data; Phosphorylation; Protein Phosphatase 1/*metabolism; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases/*metabolism; Sequence Alignment; Spindle Apparatus/metabolism; Xenopus laevis/*metabolism
    • 文献种类:期刊
    • 期刊名称: Cell
    • 期刊缩写: Cell
    • 期卷页: 2010  142 3 444-455
    • 地址: Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    • ISBN: 0092-8674
  • 相关链接 DOI URL 

  •  聚焦生物 的文献笔记  订阅


管理选项: 导出文献

评论(0 人)

facelist doodle 涂鸦板

Copyright;  © 新科学想法 2016-2017   浙公网安备 33010202000686号   ( 浙ICP备09035230号-1 )