Conditionally immortalized human podocyte cell lines established from urine
huangtingliubin 添加于 2009-12-9 11:52
| 2189 次阅读 | 0 个评论
作 者
Sakairi T, Abe Y, Kajiyama H, Bartlett LD, Howard LV, Jat PS, Kopp JB 摘 要
Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and proliferate in culture condition. To determine the phenotypic characteristics of viable urinary cells derived from human subjects, we established long-term urinary cell culture from two patients with focal segmental glomerulosclerosis (FSGS) and two healthy volunteers, via transformation with thermosensitive SV40 large T antigen (U19tsA58) together with human telomerase (hTERT). Characterization of arbitrarily selected two clonal cell lines from each human subject was carried out. mRNA expression for the podocyte markers synaptopodin, nestin, and CD2AP were detected in all eight clones. Podocin mRNA was absent from all eight clones. The expression of nephrin, Wilms tumor 1 (WT1), and podocalyxin mRNA varied among the clones, which may be due to transformation and/or cloning. These results suggest that podocyte cell lines can be established consistently from human urine. The generation of podocyte cell lines from urine of patients and healthy volunteers is novel and will help to advance studies of podocyte cell biology. Further improvements in the approaches to cell transformation and/or cell culture techniques are needed to allow cultured podocytes to fully reproduce in vivo characteristics. Key words: focal segmental glomerulosclerosis, SV40 large T antigen, human telomerase.-
详细资料
- 文献种类:期刊
- 期刊名称: American Journal of Physiology. Renal Physiology
- 期刊缩写: Am J Physiol Renal Physiol
- 期卷页: 2009年
- 地址: National Institute of Diabetes, Digestive and Kidney Diseases
- ISBN: 1522-1466
所属群组
标 签
评论( 人)
相关链接
DOI URL
浙公网安备 33010202000686号 ( 浙ICP备09035230号-1 )
临床医学专题
