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Study on the bioavailability of puerarin from Pueraria lobata isoflavone self-microemulsifying drug-delivery systems and tablets in rabbits by liquid chromatography-mass spectrometry

p535fans 添加于 2010-7-22 22:39 | 1977 次阅读 | 0 个评论

作者
Cui S, Zhao C, Tang X, Chen D, He Z
摘要
To evaluate the bioavailability of puerarin from Pueraria lobata isoflavone self-microemulsifying drug delivery systems (SMEDDS) and Yufengningxin tablets, a rapid and specific liquid chromatography--mass spectrometric method was developed and validated to determine puerarin in rabbit serum. The analyte was extracted from serum samples by precipitating the serum proteins, separated on a Diamonsil C(18) column and detected by mass spectrometry with an electrospray ionization interface. 4-Hydroxybenzaldehyde was used as the internal standard. The method has a limit of quantitation of 10 ng/mL using 200 microL serum. The intra-day relative standard deviations (RSDs) ranged from 3.7 to 6.9% and inter-day RSDs were within 6.5%. After administration of SMEDDS and tablets to rabbits, a significant difference was observed in main pharmacokinetic parameters of t(max), C(max) and AUC(0--infinity) between SMEDDS and tablets, and a 2.2-fold increase in the relative bioavailability of puerarin was observed with the SMEDDS compared with Yufengningxin tablets. It was concluded that the absorption of puerarin from Pueraria lobata isoflavone SMEDDS was enhanced.
详细资料
- 关键词: Administration, Oral; Animals; Biological Availability; Chromatography, Liquid/methods; Drug Delivery Systems; Emulsions; Isoflavones/*pharmacokinetics; Male; Mass Spectrometry; Pueraria/*chemistry; Rabbits
- 文献种类: Journal Article
- 期刊名称: Biomedical Chromatography : BMC
- 期刊缩写: Biomed Chromatogr
- 期卷页: 2005年第19卷第5期 375-378页
- 地址: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China
- ISBN: 0269-3879
- 备注:PMID:15627278
学科领域生物医药 » 药学
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