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[Study on effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats]

p535fans 添加于 2010-7-24 00:06 | 2337 次阅读 | 0 个评论

作者
Guo Y, Wan H-T, Zhang L, Wang Y, Han J, Yu Q, Pan Y-J
摘要
OBJECTIVE: To build an experimental method to determine the content of puerarin in plasma, observe the effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats suffering cerebral ischemia attack after being orally administered the main active parts of Yangyin Tongnao granules. METHOD: Absorption of puerarin in plasma was extracted by acetonitrile. Using hydroxybenzoate as interior standard, methanol-0.5% acetic acid water solution as mobile phase, ODS C(18) chromatography column (4.6 mm x 150 mm, 5 microm) as stationary phase, puerarin was detected at 248 nm. RESULT: There is a good linear relationship for puerarin in the range 0.025-3.2 microg x mL-1, RSD < 10%, and the average recovery is 97.6%. CONCLUSION: The method is sensitive, reliable, and can be well applied in this experiment. It can provide a method for determination of active components in TCM preparation in vitro. Different compatibility influences metabolism of components.
详细资料
- 关键词: Alkaloids/chemistry/isolation & purification; Animals; Area Under Curve; Brain Ischemia/blood; Drug Combinations; *Drug Interactions; Drugs, Chinese Herbal/administration & dosage/chemistry/*pharmacology; Flavones/chemistry/isolation & purification; Isoflavones/*blood/metabolism/*pharmacokinetics; Male; Oils, Volatile/chemistry/isolation & purification; Plants, Medicinal/chemistry; Pueraria/chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; Saponins/chemistry/isolation &am
- 文献种类: Journal Article
- 期刊名称: Zhongguo Zhong yao za zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
- 期刊缩写: Zhongguo Zhong Yao Za Zhi
- 期卷页: 2007年第32卷第23期 2531-2534页
- 地址: Institute of Heart Brain Vessel Disease, Zhejiang Chinese Medical University, Hangzhou 310053, China
- ISBN: 1001-5302
- 备注:PMID:18330251
学科领域生物医药 » 药学
标签

puerarin metabolism rats prescription
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