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Selecting an "orthogonal" column during high-performance liquid chromatographic method development for samples that may contain non-ionized solutes

luckyman 添加于 2009-3-30 06:47 | 2157 次阅读 | 0 个评论

作者
Dolan JW, Snyder LR
摘要
Several reasons can exist for a change in reversed-phase selectivity, and several separation conditions can be changed for this purpose. In the present investigation, a change in column is considered for the improved separation of non-ionized solutes only. Differences in column selectivity (and the selection of "orthogonal" columns) can be predicted on the basis of the hydrophobic-subtraction model. For ionized solutes, the F(s)-parameter is used to predict orthogonality. For use with non-ionized solutes, it is suggested that the cation-exchange term of the model be dropped [F(s)(-C)] for better predictions. For samples containing both ionized and non-ionized solutes, F(s) and F(s)(-C) should be used together for the best results. Predicted separations involving 64 non-ionized solutes and maximally different columns from a 400-column database were used to validate this procedure.
详细资料
- 文献种类: Journal Article
- 期刊名称: Journal of Chromatography. A
- 期卷页: 2008年
- 地址: LC Resources Inc., 15664 SE Woodland Hts, Amity, OR 97101, USA
- ISBN: 0021-9673
学科领域生物医药 » 药学
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