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Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis

阿平添加于 2010-10-7 01:16 | 2841 次阅读 | 0 个评论

作者
Posch M, Khoudoli GA, Swift S, King EM, Deluca JG, Swedlow JR
摘要
We have studied Sds22, a conserved regulator of protein phosphatase 1 (PP1) activity, and determined its role in modulating the activity of aurora B kinase and kinetochore-microtubule interactions. Sds22 is required for proper progression through mitosis and localization of PP1 to mitotic kinetochores. Depletion of Sds22 increases aurora B T-loop phosphorylation and the rate of recovery from monastrol arrest. Phospho-aurora B accumulates at kinetochores in Sds22-depleted cells juxtaposed to critical kinetochore substrates. Sds22 modulates sister kinetochore distance and the interaction between Hec1 and the microtubule lattice and, thus, the activation of the spindle assembly checkpoint. These results demonstrate that Sds22 specifically defines PP1 function and localization in mitosis. Sds22 regulates PP1 targeting to the kinetochore, accumulation of phospho-aurora B, and force generation at the kinetochore-microtubule interface.
详细资料
- 文献种类: Journal Article
- 期刊名称: The Journal of Cell Biology
- 期刊缩写: J Cell Biol
- 期卷页: 2010年第191卷第1期 61-74页
- 地址: Wellcome Trust Centre for Gene Regulation and Expression and 2 Light Microscopy Facility, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
- ISBN: 0021-9525
- 备注:PMID:20921135
学科领域生物医药 » 生物学
标签

Sds22
相关链接 DOI URL

此文一出，心头的一块石头落下了，本来还打算做完现在这个项目就做这个的，现在答案已经有了，还好没有话费太多时间在这个上面。

不过显然这篇文章还有缺陷，那就是主要描述了Sds22和Aurora B的关系，缺乏另一大主角PP1束之高阁，而且蛋白酶底物也没有予以描述。

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