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Regulation of mitotic chromosome cohesion by Haspin and Aurora B

阿平添加于 2009-5-24 21:48 | 3000 次阅读 | 0 个评论

作者
Dai J, Sullivan BA, Higgins JMG
摘要
In vertebrate mitosis, cohesion between sister chromatids is lost in two stages. In prophase and prometaphase, cohesin release from chromosome arms occurs under the control of Polo-like kinase 1 and Aurora B, while Shugoshin is thought to prevent removal of centromeric cohesin until anaphase. The regulatory enzymes that act to sustain centromeric cohesion are incompletely described, however. Haspin/Gsg2 is a histone H3 threonine-3 kinase required for normal mitosis. We report here that both H3 threonine-3 phosphorylation and cohesin are located at inner centromeres. Haspin depletion disrupts cohesin binding and sister chromatid association in mitosis, preventing normal chromosome alignment and activating the spindle assembly checkpoint, leading to arrest in a prometaphase-like state. Overexpression of Haspin hinders cohesin release and stabilizes arm cohesion. We conclude that Haspin is required to maintain centromeric cohesion during mitosis. We also suggest that Aurora B regulates cohesin removal through its effect on the localization of Shugoshin.
详细资料
- 关键词: Cell Cycle Proteins/genetics/*physiology; Cell Line, Tumor; Centromere/genetics/physiology; Chromatids/genetics/physiology; Chromosomal Proteins, Non-Histone/physiology; Chromosomes, Human/genetics/*physiology; Histones/genetics/physiology; Humans; Intracellular Signaling Peptides and Proteins; *Mitosis; Nuclear Proteins/physiology; Phosphorylation; Protein-Serine-Threonine Kinases/genetics/*physiology
- 文献种类: Journal Article
- 期刊名称: Developmental Cell
- 期刊缩写: Dev Cell
- 期卷页: 2006年第11卷第5期 741-750页
- 地址: Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham & Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
- ISBN: 1534-5807
- 备注:PMID:17084365
学科领域生物医药 » 生物学
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附件
Regulation of mitotic chromosome cohesion by Haspin and Aurora B