Long noncoding RNA as modular scaffold of histone modification complexes
biocq 添加于 2012-3-25 14:26
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作 者
Tsai M-C, Manor O, Wan Y, Mosammaparast N, Wang JK, Lan F, Shi Y, Segal E, Chang HY 摘 要
Long intergenic noncoding RNAs (lincRNAs) regulate chromatin states and epigenetic inheritance. Here, we show that the lincRNA HOTAIR serves as a scaffold for at least two distinct histone modification complexes. A 5' domain of HOTAIR binds polycomb repressive complex 2 (PRC2), whereas a 3' domain of HOTAIR binds the LSD1/CoREST/REST complex. The ability to tether two distinct complexes enables RNA-mediated assembly of PRC2 and LSD1 and coordinates targeting of PRC2 and LSD1 to chromatin for coupled histone H3 lysine 27 methylation and lysine 4 demethylation. Our results suggest that lincRNAs may serve as scaffolds by providing binding surfaces to assemble select histone modification enzymes, thereby specifying the pattern of histone modifications on target genes.-
详细资料
- 关键词: Binding Sites; Carrier Proteins/metabolism; Cell Line; Cells, Cultured; Chromatin/*metabolism; Chromatin Immunoprecipitation; DNA-Binding Proteins/*metabolism; HeLa Cells; Histone Demethylases/*metabolism; Histones/*metabolism; Humans; Methylation; Mutation; Nerve Tissue Proteins/metabolism; Nuclear Proteins/metabolism; Nucleic Acid Conformation; Promoter Regions, Genetic; Protein Binding; RNA Interference; RNA, Untranslated/chemistry/*metabolism; Repressor Proteins/*metabolism; Transcription Fa ...
- 文献种类: Journal Article
- 期刊名称: Science (New York, N.Y.)
- 期刊缩写: Science
- 期卷页: 2010年 第329卷 第5992期 689-693页
- 地址: Howard Hughes Medical Institute and Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
- ISBN: 0036-8075
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Long noncoding RNA as modular scaffold of histone modification complexes
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