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有读书笔记有附件Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase

聚焦生物 添加于 2012-12-26 21:41 | 2295 次阅读 | 0 个评论

  •  作 者

    Buganim Y, Faddah DA, Cheng AW, Itskovich E, Markoulaki S, Ganz K, Klemm SL, van Oudenaarden A, Jaenisch R

  •  摘 要

    During cellular reprogramming, only a small fraction of cells become induced pluripotent stem cells (iPSCs). Previous analyses of gene expression during reprogramming were based on populations of cells, impeding single-cell level identification of reprogramming events. We utilized two gene expression technologies to profile 48 genes in single cells at various stages during the reprogramming process. Analysis of early stages revealed considerable variation in gene expression between cells in contrast to late stages. Expression of Esrrb, Utf1, Lin28, and Dppa2 is a better predictor for cells to progress into iPSCs than expression of the previously suggested reprogramming markers Fbxo15, Fgf4, and Oct4. Stochastic gene expression early in reprogramming is followed by a late hierarchical phase with Sox2 being the upstream factor in a gene expression hierarchy. Finally, downstream factors derived from the late phase, which do not include Oct4, Sox2, Klf4, c-Myc, and Nanog, can activate the pluripotency circuitry.

  •  详细资料

    • 关键词: Animals; Cell Line; Embryo, Mammalian/cytology; Embryonic Stem Cells; Fibroblasts/cytology/metabolism; Genetic Markers; Induced Pluripotent Stem Cells/cytology/*metabolism; Mice; Microfluidic Analytical Techniques; *Nuclear Reprogramming; SOXB1 Transcription Factors/metabolism; *Single-Cell Analysis; Transcription Factors/metabolism; *Transcriptome
    • 文献种类: Journal Article
    • 期刊名称: Cell
    • 期刊缩写: Cell
    • 期卷页: 2012年  第150卷 第6期 1209-1222页
    • 地址: The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    • ISBN: 0092-8674

  • 学科领域 生物医药 » 生物学

  •  所属群组

    生物综合  

  • 相关链接 DOI URL 

  •  附 件

    PDF附件Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase 

  •  聚焦生物 的文献笔记  订阅

    来自美国麻省理工学院怀特海德研究所的研究人员鉴定出新的基因标记物,从而可能有助于提高重编程效率和允许科学家们预测哪些经过基因修饰的细胞将成功地变 成多能性的干细胞.他们还鉴定出新的重编程因子组合来产生iPSCs. 这项研究也是第一次研究当单个细胞变成iPSCs时,它们所发生的基因变化.以前的研究只观察大的细胞群体---实际上并不是所有的细胞都能够发生重编程 ---在经历重编程过程中所发生的基因表达变化,这就使得科学家们很难挑选出参与这种过程的基因
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