新科学想法 › 学术文库 › 学术文献 › 浏览文献

Circular transcripts of the testis-determining gene Sry in adult mouse testis

热1 liufzhq 添加于 2013-3-30 12:49 | 9640 次阅读 | 1 个评论

作者
Capel B, Swain A, Nicolis S, Hacker A, Walter M, Koopman P, Goodfellow P, Lovell-Badge R
摘要
Sry is expressed at higher levels in the adult testis, where no function has been determined, than in the genital ridge, its critical site of action. cDNA and 5\' RACE clones isolated from testis or from Sry-transfected cell lines have an unusual structure, with 3\' sequences located in a 5\' position. RNAase protection assays and reverse transcription polymerase chain reactions confirmed that these unusual RNA molecules represent the most abundant transcript in testis. Furthermore, oligonucleotide hybridization and RNAase H digestion proved that these Sry RNA molecules are circular. Similar transcripts were detected in the testes of mice with Mus musculus musculus, Mus musculus domesticus, and Mus spretus Sry genes. The circular RNA is found in the cytoplasm but is not substantially bound to polysomes. We suggest that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
详细资料
- 关键词: Animals; Base Sequence; Cloning, Molecular; DNA-Binding Proteins/*genetics; Gene Library; Male; Mice; Molecular Sequence Data; Muridae; *Nuclear Proteins; Nucleic Acid Conformation; Organ Specificity; Polymerase Chain Reaction; RNA/*analysis; RNA, Messenger/*analysis; Ribonuclease H; Ribonuclease T1; Ribonuclease, Pancreatic; Sex-Determining Region Y Protein; Species Specificity; Testis/*chemistry; *Transcription Factors
- 文献种类: Journal Article
- 期刊名称: Cell
- 期刊缩写: Cell
- 期卷页: 1993年第73卷第5期 1019-1030页
- 地址: Laboratory of Eukaryotic Molecular Genetics, Medical Research Council, National Institute for Medical Research, London, England
- ISBN: 0092-8674
学科领域生物医药 » 生物学
相关链接 URL