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[Tobacco--a producer of recombinant interleukins]

xuehongbai 添加于 2014-1-31 23:40 | 1175 次阅读 | 0 个评论

作者
Budzianowski J
摘要
Interleukins are cytokines of highly pleiotropic activity and they have high potential for application in the treatment of cancer and autoimmune diseases. Trials of recombinant interleukin production in plants relate almost exclusively to tobacco, where through the transformation of the nuclear genome (agroinfection) monomeric (IL-2, IL-4, IL-13, IL-18), homodimeric (IL-10) and single-chain heterodimeric (IL-12) interleukins have been obtained. The expression of IL-10 as a homodimer in the chloroplast genome could not be reached. Expression of the given interleukin was obtained in the leaves, cell culture and culture of hairy roots of tobacco. Interleukins obtained in tobacco showed similar in vitro biological activity as commercial ILs produced mostly in E. coli. Glycosylated IL-13 obtained in tobacco was much more resistant to proteolytic digestion than commercial non-glycosylated IL-13; therefore in the case of sufficiently large production it could be suitable for oral administration in the treatment of type I diabetes.
详细资料
- 关键词: Animals; Cell Culture Techniques; Diabetes Mellitus, Type 1/drug therapy; Escherichia coli/metabolism; Humans; Interleukin-12/biosynthesis; Interleukin-13/biosynthesis/therapeutic use; Interleukin-18/biosynthesis; Interleukin-2/biosynthesis; Interleukin-4/biosynthesis; Interleukins/*analysis/*biosynthesis; Plant Leaves/chemistry/metabolism; Recombinant Proteins/*analysis/*biosynthesis; Tobacco/*chemistry/*metabolism
- 文献种类: Journal Article
- 期刊名称: Przeglad Lekarski
- 期刊缩写: Przegl Lek
- 期卷页: 2012年第69卷第10期 1060-1062页
- 地址: Katedra i Zaklad Botaniki Farmaceutycznej i Biotechnologii Roslin, Uniwersytet Medyczny im. Karola Marcinkowskiego, 61-861 Poznan, ul. Sw. Marii Magdaleny 14. jbudzian@ump.edu.pl
- ISBN: 0033-2240
学科领域生物医药 » 药学
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