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High-performance liquid chromatography separation of cis-trans anthocyanin isomers from wild Lycium ruthenicum Murr. employing a mixed-mode reversed-phase/strong anion-exchange stationary phase

晓龙添加于 2016-4-15 18:13 | 1315 次阅读 | 0 个评论

作者
Jin H, Liu Y, Guo Z, Yang F, Wang J, Li X, Peng X, Liang X
摘要
The cis-trans isomerism is a common phenomenon for acylated anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure anthocyanins, including two pairs of cis-trans isomeric anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate anthocyanins, especially for cis-trans isomers.
详细资料
- 关键词: Adsorption; Anion Exchange Resins/chemistry; Anthocyanins/chemistry/*isolation & purification; Chromatography, High Pressure Liquid/instrumentation/*methods; Chromatography, Ion Exchange/instrumentation/*methods; Isomerism; Lycium/*chemistry; Plant Extracts/chemistry/*isolation & purification; Hplc; Lycium ruthenicum Murr.; anthocyanins; cis-trans anthocyanin isomer; mixed-mode stationary phase
- 文献种类: Journal Article
- 期刊名称: Journal of Agricultural and Food Chemistry
- 期刊缩写: J Agric Food Chem
- 期卷页: 2015年第63卷第2期 500-508页
- 地址: State Key Laboratory of Fine Chemicals, Dalian University of Technology , Dalian, Liaoning 116012, People\'s Republic of China
- ISBN: 0021-8561
学科领域自然科学 » 化学
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