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Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column

晓龙添加于 2016-4-15 18:13 | 1292 次阅读 | 0 个评论

作者
Li X, Guo Z, Wang C, Shen A, Liu Y, Zhang X, Zhao W, Liang X
摘要
As a kind of promising anticancer compounds, the preparation of bufadienolides is a hot study spot. However, due to the complexity of biological sample, the purification of bufadienolides from a crude sample (toad skin) is a tough work. In this paper, we reported a new way based on positively charged C18 material (XCharge C18) to quickly separate and purify bufadienolides from toad skin. By this method, the different ionic feature of the amino acid conjugated bufadienolides (AACBs) and the free form bufadienolides (AAUBs) was firstly utilized to obtain distinct separation selectivity on the XCharge C18 column. Additionally, the peak tailing problem of AACBs on conventional C18 was resolved and better resolutions were achieved on the XCharge C18, thus, two kinds of bufadienolides on one column were successfully purified respectively. Taking F13 as an example, the method was validated by liquid chromatography-mass spectrometry (LC-MS), and then 4 AACBs as well as 4 AAUBs were simultaneously purified by preparative XCharge C18. In addition, the application of this method in other fractions was also validated. The results suggested that the developed method is a practical and promising tool for efficient separation and purification of bufadienolides from toad skin.
详细资料
- 关键词: Animals; Bufanolides/*chemistry; Bufo bufo/*metabolism; Chromatography, High Pressure Liquid/methods; Mass Spectrometry/methods; Pyridazines/*chemistry; Skin/*chemistry; Bufadienolides; Bufo bufo gargarizans Cantor; Positively charged C18; Purification; Reversed-phase liquid chromatography
- 文献种类: Journal Article
- 期刊名称: Journal of Pharmaceutical and Biomedical Analysis
- 期刊缩写: J Pharm Biomed Anal
- 期卷页: 2014年第92卷 105-113页
- 地址: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: liangxm@dicp.ac.cn
- ISBN: 0731-7085
学科领域自然科学 » 化学
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