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Augmentation of miR-202 in varicose veins modulates phenotypic transition of vascular smooth muscle cells by targeting proliferator-activated receptor-gamma coactivator-1alpha

我爱微笑添加于 2018-12-31 21:53 | 993 次阅读 | 0 个评论

作者
Huang X, Liu Z, Shen L, Jin Y, Xu G, Zhang Z, Fang C, Guan W, Liu C
摘要
In varicose veins, vascular smooth muscle cells (VSMCs) often show abnormal proliferative and migratory rates and phenotypic transition. This study aimed to investigate whether microRNA (miR)-202 and its potential target, peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha), were involved in VSMC phenotypic transition. miR-202 expression was analyzed in varicose veins and in VSMCs conditioned with platelet-derived growth factor. The effect of miR-202 on cell proliferation and migration was assessed. Furthermore, contractile marker SM-22alpha, synthetic markers vimentin and collagen I, and PGC-1alpha were analyzed by Western blot analysis. The modulation of PGC-1alpha expression by miR-202 was also evaluated. In varicose veins and proliferative VSMCs, miR-202 expression was upregulated, with decreased SM-22alpha expression and increased vimentin and collagen I expression. Transfection with a miR-202 mimic induced VSMC proliferation and migration, whereas a miR-202 inhibitor reduced cell proliferation and migration. miR-202 mimic constrained luciferase activity in HEK293 cells that were cotransfected with the PGC-1alpha 3\'-untranslated region (3\'-UTR) but not those with mutated 3\'-UTR. miR-202 suppressed PGC-1alpha protein expression, with no influence on its messenger RNA expression. PGC-1alpha mediated VSMC phenotypic transition and was correlated with reactive oxygen species production. In conclusion, miR-202 affects VSMC phenotypic transition by targeting PGC-1alpha expression, providing a novel target for varicose vein therapy.
详细资料
- 关键词: microRNA-202; peroxisome proliferator-activated receptor-gamma coactivator-1alpha; phenotypic transition; varicose veins; vascular smooth muscle cells
- 文献种类: Journal Article
- 期刊名称: Journal of Cellular Biochemistry
- 期刊缩写: J Cell Biochem
- 期卷页: 2018年
- 地址: Department of Vascular Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, Jiangsu, China
- ISBN: 0730-2312
学科领域生物医药 » 临床医学
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