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有读书笔记Phosphorylation of serine-10 of histone H3 shields modified lysine-9 selectively during mitosis

阿平 添加于 2010-1-16 07:31 | 3181 次阅读 | 0 个评论

  •  作 者

    Jeong YS, Cho S, Park JS, Ko Y, Kang YK

  •  摘 要

    Post-translational modifications of histones play important roles in regulating chromatin dynamics and epigenetic inheritance during mitosis. The epigenetic significance and stability of histone H3-lysine 9 (H3K9) modifications have been well studied in interphase cells, whereas not as much in mitotic cells. Here, we inspected mitosis-coupled alterations in the global modifications of H3K9. Signals for H3K9 mono-, di-methylation and acetylation became invisible as cells entered mitosis in contrast to the pattern observed for H3-serine 10 phosphorylation (H3S10ph). Treatment with the aurora-B inhibitor ZM447439 or expression of the dominant negative mutant Aur-B(K106R) resulted in prometaphase chromosomes that lacked signals for H3S10ph but were positive for H3K9 modifications. Trimethylation was the sole K9 modification that remained consistently detectable throughout the cell cycle. This phenomenon was specific for H3K9-S10, as this pattern was not observed at H3K27-S28. Methylated H3K27 remained detectable throughout the cell cycle, despite phosphorylation of the adjacent H3S28. Contrastingly, our dot-blot experiment using synthetic peptides showed that phosphorylation of serine residue basically kept adjacent lysine from antibody access. Together, these results suggest that phosphorylation of serine residue occurs in a selective manner, being influenced by the types of modifications and the nature of neighboring lysine residues.

  •  详细资料

    • 文献种类: Journal Article
    • 期刊名称: Genes to Cells : Devoted to Molecular & Cellular Mechanisms
    • 期刊缩写: Genes Cells
    • 期卷页: 2010年
    • 地址: Development and Differentiation Research Center, KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea
    • ISBN: 1365-2443
    • 备注:PMID:20070858

  • 学科领域 生物医药 » 生物学

  •  所属群组

    表遗传   蛋白组学   细胞周期  

  •  标 签

    H3S10  H3K9 

  • 相关链接 DOI URL 

  •  阿平 的文献笔记  订阅

    组蛋白相邻位点的互动到底是怎么回事？

    在有丝分裂期间，H3K9me\me2\ac的信号全部消失，此时H3S10ph信号占据主导地位，鉴于其两个位点的临近关系，作者认为H3S10ph可以让H3K9相关抗体无法识别K9，并且用磷酸化的合成肽来应征这种推论。然后同样是临近关系，H3K27和H3S28ph则不会有这种互相干扰的关系

    说实话，这种观点我在以前的一篇文章中早就看到过了，此文不算什么新意，而且这种推论已经被证明是有错误的。

    因为太多的例子证明，抗体对此类组蛋白修饰的识别是以固定pattern出现的。比如有些抗体可以识别H3K9me和H3S10ph共同存在的情况，但是不能识别其他。那么这样的抗体就是识别特定pattern的抗体，而不是说光光一个位点的。

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