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有读书笔记有附件Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes

阿平 添加于 2009-1-20 05:55 | 3109 次阅读 | 0 个评论
  •  作 者

    Hsu JY, Sun ZW, Li X, Reuben M, Tatchell K, Bishop DK, Grushcow JM, Brame CJ, Caldwell JA, Hunt DF, Lin R, Smith MM, Allis CD
  •  摘 要

    Phosphorylation of histone H3 at serine 10 occurs during mitosis and meiosis in a wide range of eukaryotes and has been shown to be required for proper chromosome transmission in Tetrahymena. Here we report that Ipl1/aurora kinase and its genetically interacting phosphatase, Glc7/PP1, are responsible for the balance of H3 phosphorylation during mitosis in Saccharomyces cerevisiae and Caenorhabditis elegans. In these models, both enzymes are required for H3 phosphorylation and chromosome segregation, although a causal link between the two processes has not been demonstrated. Deregulation of human aurora kinases has been implicated in oncogenesis as a consequence of chromosome missegregation. Our findings reveal an enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes.
  •  详细资料

    • 关键词: Animals; Caenorhabditis elegans/*cytology/metabolism; Fungal Proteins/*metabolism; Genome; Helminth Proteins/metabolism; Histones/*metabolism; *Mitosis; Phenotype; Phosphoprotein Phosphatases/*metabolism; Phosphorylation; Protein-Serine-Threonine Kinases/*metabolism; RNA, Antisense; RNA, Small Interfering; Saccharomyces cerevisiae/*cytology/metabolism; Serine/metabolism; Species Specificity
    • 文献种类: Journal Article
    • 期刊名称: Cell
    • 期卷页: 2000  102 3 279-291
    • 地址: Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville 22908, USA
    • ISBN: 0092-8674
  • 学科领域 生物医药 » 生物学

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  •  阿平 的文献笔记  订阅

    酵母菌和线虫模型

    证明GLC7(PP1)为H3S10ph的对应磷酸酶的方法:

    1.突变GLC7,结果H3S10ph增强

    2.invitro assay:核小体+ 32P-标记 + PKA = H3S10ph;用IP得到HA-glc7,然后混合测试invitro dephosphorylation

    3.Glc7和aurora kinase 互相对立,double RNAi可以resue phenotype

     

    评论:

    不足,没有in vivo数据支持,最好能够证明PP1就是sit 在histone上,或者PP1的regulator也行。

    之考虑了kinase和phopatase的对立作用,没有考虑regulator潜在的target作用。

    借鉴之处:在考虑针对kinase产生的摸个phenotype的时候,要充分考虑到该kinase可能有很多种底物,不一定你考虑的底物就是主要原因。

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